List of top Biotechnology Questions

If there are three unrooted trees for four protein sequences, the number of rooted trees for the same number of sequences is ________________.

Aqueous two-phase extraction is used to recover \(\alpha\)-amylase from a solution. A polypropylene glycol–dextran mixture is added and the solution separates into upper and lower phases. The partition coefficient is 4.0 and the ratio of upper to lower phase volume is 5.0. The enzyme recovery or yield (in percentage, rounded off to the nearest integer) is ____________________.

Temperature of a reaction with an activation energy value of 15 kcal mol\(^{-1}\) is increased from 300 K to 310 K. If the value of the ideal gas constant (R) is 1.9872 cal mol\(^{-1}\) K\(^{-1}\), the ratio of the reaction rate constants \(\left(\frac{k_{310}}{k_{300}}\right)\) (rounded off to two decimal places) is ____________.

The following reaction represents biomass synthesis from hexadecane:
\(\,C_{16}H_{34} + 12.5 O_2 + 2.13 NH_3 \rightarrow 10.6 CH_{1.66}O_{0.27}N_{0.27} + 5.37 CO_2 + 11.4 H_2O \)
where \( CH_{1.66}O_{0.27}N_{0.27} \) represents the biomass. The value of respiratory quotient (rounded off to two decimal places) is ________________.

Direct DNA transfer method(s) used for plant genetic engineering is(are)

Which of the following vector(s) is(are) used to clone a DNA fragment of size 220 kb?

Methylation of CpG islands near the promoter of a gene can inhibit transcription by

Which of the following statement(s) is(are) TRUE about induced pluripotent stem cells?

The event(s) that lead(s) to inactivation of tumor suppressor genes in cancer cells is(are)

Which of the following is(are) plant protoplast fusogenic agent(s)?

Which one of the following methods CANNOT be used to determine the secondary structure content of a protein?

Fabry disease in humans is a X-linked disease. The probability (in percentage) for a phenotypically normal father and a carrier mother to have a son with Fabry disease is ________________.

Which one of the following programs is used for finding distantly related (or remote) protein homologs?

Which one of the following is used for global alignment of two protein sequences?

Which one of the following plant growth regulators facilitate adventitious root formation?

In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?
P. Alkaline phosphatase
Q. Trypsinase
R. Horseradish peroxidase
S. Amylase

Which of the following methods are used for detection of DNA and RNA, respectively?

In hybridoma technology, which one of the following enzymes is absent in the myeloma cells that are used for monoclonal antibody production?

One of the following is correct match or mechanisms select it :

Which of the following characteristics is most likely to be associated with a high apparent volume ofdistribution?

Match List I and List II

	List I: Fermentation Products		List II: Strain used
A	Mast cells	I	Clostridium tetani
B	Lymphocytes	II	Brevibacterium sp.
C	T-cells	III	Leuconostac mesenteroids
D	Monocytes- Macrophages	IV	Bacillus subtillis
		V	Streptomyces olivaceus

Choose the correct answer from the options given below:

The total number of amino acids in human insulin (total number of amino acids in A and B chain) is

Which one of the following is not a gene insertion technique into a eukaryotic cell

Pyrogens present in containers can be destroyed by heating the containers at: