List of top Biotechnology Questions

Assertion (A): The exact chemical composition of complex microbial growth media is known.
Reason (R): Complex nutrient media is used when specific growth requirement of a microorganism is unknown.
When a transgene from a Genetically Modified crop escapes through pollen to a related plant species, it is known as:
Embryonic stem cells derived from the blastocyst stage of the embryo are ________________________ in nature.
An example of secondary metabolites produced by microbial cells include:
A protein ion with a molecular weight of 10,000 Daltons carried a charge of 5+ and was subjected to mass spectrometric analysis. Calculate its mass to charge ratio.
Artificial seeds are produced by encapsulating the somatic embryos at the ______________ stage in a protective coating.
Severe combined immunodeficiency disease is caused due to the absence of:
Single nucleotide polymorphisms usually occur in ______________ regions.
Identify the vector that infects E. coli cells containing F-plasmid and that has a single-stranded circular genome:}
The peptide hormones and growth factors to promote healthy growth of animal cells in vitro are often derived from:}
The natural source of enzyme barnase and barstar, a system used to achieve male sterile plant is:
Virus-mediated transfer of host DNA from one cell to another cell is known as:
The Phase in which two identical copies of DNA are formed is:
Virus mediated transfer of host DNA from one cell to another cell is known as:
The Phase in which two identical copies of DNA are formed is:

E. coli cultivated at 298 K uptakes an uncharged compound (A) by passive diffusion. The intracellular and extracellular concentrations of A are 0.001 M and 0.1 M, respectively. If \(R=1.9872\ \mathrm{cal\ mol^{-1}\ K^{-1}}\), the free-energy change (in \(\mathrm{kcal\ mol^{-1}}\)) for this passive diffusion of A (rounded off to two decimal places) is ________________.

E. coli is cultivated in a chemostat operated at a dilution rate of 0.2 h\(^{-1}\). The values of biomass yield due to oxygen consumption and the steady state biomass concentration are 0.2 g g\(^{-1}\) and 10 g L\(^{-1}\), respectively. The oxygen transfer rate (in g L\(^{-1}\) h\(^{-1}\)) is ____________.

A dNTP master-mix is prepared by combining \(40~\mu\text{L}\) of each \(20~\text{mM}\) dNTP stock (dATP, dCTP, dGTP and dTTP). \(4~\mu\text{L}\) of this dNTP master-mix is added to a PCR mix and the final volume is adjusted to \(50~\mu\text{L}\). The concentration (in \(\mu\text{M}\)) of total dNTPs in the PCR mix is ________________.
A proportional controller is used to control the temperature of an autoclave from \(60^{\circ}C\) to \(130^{\circ}C\). If the proportional band setting of the controller is 25%, the proportional gain value is ______________.
An evaporator is insulated using glass wool material of \(0.15\ \text{m}\) thickness. The inner most surface and the outer surface of the insulation are at \(700^{\circ}\text{C}\) and \(80^{\circ}\text{C}\), respectively. The mean thermal conductivity of the glass wool under these conditions is \(0.29\ \text{W}\,\text{m}^{-1}\,\text{K}^{-1}\). The rate of heat loss (in W) through \(1.2\ \text{m}^2\) of the evaporator wall surface (rounded off to the nearest integer) is ________________.
The values of the consistency index ‘\(K\)’ and the flow behavior index ‘\(n\)’ of a dilatant fluid are 0.415 (in CGS units) and 1.23, respectively. The value of the apparent viscosity (in \( g \cdot cm^{-1} \cdot s^{-1} \)) of this fluid at a shear rate of 60 \( s^{-1} \) (rounded off to the nearest integer) is __________________.
A protein has three identical sites arranged at the vertices of an equilateral triangle. With one site filled with donor dye, the quantum yield \(\phi_D\) is \(0.5\). Filling one site with donor and a second with acceptor gives \(\phi_D=0.25\). Find \(\phi_D\) when one site has donor and the other two have acceptor dyes (rounded to three decimals).
If \(1000\) bp of a double-helical DNA weighs \(1\times10^{-18}\) g and the distance between two bp is \(0.34~\text{nm}\), the total amount of DNA (in mg, rounded to one decimal) required to stretch from Earth to Moon (distance \(=3.74\times10^{5}\) km) is ________________.
The following schematic diagram shows a chemostat with cell recycle (feed flow rate \(F_0\) and recycle flow rate \(F_r\) in \(\mathrm{L\,h^{-1}}\)). The cell concentrations in the reactor, recycle stream, and product stream are \(X_1\), \(X_0\), and \(X\) (in \(\mathrm{g\,L^{-1}}\)), respectively. If \(\dfrac{X_0}{X_1}=1.5\), \(\dfrac{F_r}{F_0}=0.7\), and \(X_1=7.3~\mathrm{g\,L^{-1}}\), the value of \(X\) (in \(\mathrm{g\,L^{-1}}\), rounded off to one decimal place) is ________________.
DNA sample collected from an unidentified bacterial species (Y) contains \(13%\) adenine. The \(G{+}C\) content (in percentage) of Y is ________________.