Question

The most important use of BAC vector is for:

• A. Stable transfection
• B. Human genomic library construction
• C. E. coli protein expression
• D. E. coli genomic library construction

Hint:

BAC vectors are used to clone large DNA fragments, making them ideal for constructing genomic libraries.

Answer 1

The Correct Option is B

Step 1: Understanding BAC Vectors 
Bacterial artificial chromosomes (BACs) are large cloning vectors used for cloning large DNA fragments, typically up to 300 kb. 
Step 2: Evaluating the Options 
- Stable transfection: Not the primary use of BACs. 
- Human genomic library construction: Correct, BAC vectors are ideal for constructing large human genomic libraries. 
- E. coli protein expression: E. coli protein expression typically uses plasmid vectors, not BACs. 
- E. coli genomic library construction: Not typically used for constructing genomic libraries; BACs are better for large DNA fragments. 
Step 3: Conclusion 
The most important use of BAC vectors is for constructing human genomic libraries.