Question

Which of the following methods can be used to detect single nucleotide change in DNA?

• A. ELISA
• B. WESTERN Blotting
• C. SDS-PAGE
• D. PCR

Hint:

PCR can detect single nucleotide changes by amplifying target DNA regions and analyzing sequence variations.

Answer 1

The Correct Option is D

Step 1: Understanding the Methods 
- ELISA: A technique used for detecting and quantifying soluble substances like proteins or antibodies, not suitable for detecting nucleotide changes. 
- WESTERN Blotting: Used for detecting proteins, not nucleotides. 
- SDS-PAGE: A technique for protein separation, not used for detecting DNA sequence changes. 
- PCR: Correct, PCR can be used to detect single nucleotide changes by amplifying specific DNA regions and comparing the sequences. 
Step 2: Conclusion 
PCR is the most suitable method for detecting single nucleotide changes in DNA.