Question

Which separation technique uses a ligand to purify its receptor protein?

• A. Ion exchange chromatography
• B. Expanded Bed Adsorption chromatography
• C. Affinity chromatography
• D. Size-exclusion chromatography

Hint:

Affinity chromatography uses a specific ligand to bind and purify a target protein.

Answer 1

The Correct Option is C

Step 1: Understanding Affinity Chromatography 
Affinity chromatography uses a ligand (specific molecule) that binds to the target protein, allowing selective purification. 
Step 2: Evaluating the Options 
- Ion exchange chromatography: Separates based on charge, not ligand binding. 
- Expanded Bed Adsorption chromatography: Uses adsorption for separation, but not ligand-based. 
- Affinity chromatography: Correct, uses ligands to selectively purify proteins. 
- Size-exclusion chromatography: Separates based on size, not ligand affinity. 
Step 3: Conclusion 
Affinity chromatography is the method that uses a ligand for selective purification of proteins.