Question

Why is the enhancer region included in many vectors based on alphaviruses?

• A. Expression of protein in N terminus
• B. Expression of protein on P terminus
• C. Expression as fusion protein
• D. To decrease the expression

Hint:

Enhancer regions in alphavirus vectors are included to increase the expression of fusion proteins.

Answer 1

The Correct Option is C

Step 1: Understanding Enhancer Regions in Alphavirus Vectors 
Enhancer regions are included in viral vectors, such as alphaviruses, to boost the expression of recombinant genes. 
Step 2: Evaluating the Options 
- Expression of protein in N terminus: Not the main function of enhancer regions. 
- Expression of protein on P terminus: Also not related to the enhancer region's function. 
- Expression as fusion protein: Correct, enhancer regions help in high-level expression, often used in fusion protein expression. 
- To decrease the expression: Enhancer regions are used to increase, not decrease, gene expression. 
Step 3: Conclusion 
Enhancer regions are included to increase protein expression, especially when expressed as fusion proteins.