List of top Biotechnology Questions

Compare the techniques of FISH with Microarray in terms of principle and applications.

Give any two drawbacks of animal cell culture in vitro.}

Name the technique that helps to study the entire protein profile from a given cell type. Briefly explain the principle of this method.

Assertion (A): The exact chemical composition of complex microbial growth media is known.
Reason (R): Complex nutrient media is used when specific growth requirement of a microorganism is unknown.

Assertion (A): During plant tissue culture, the explants are treated with sodium hypochlorite.
Reason (R): Sodium hypochlorite helps in acclimatization of the regenerated plants.

Assertion (A): Some experts believe that there must be more than 30,000 genes in human genome.
Reason (R): Unreliability of in silico gene prediction is responsible for reporting lesser number of genes in human genome.

________________________ cultures can be maintained for a prolonged period of time by repeated sub-culturing.

Assertion (A): The regulation of pH is essential for survival of mammalian cells.
Reason (R): Animal cell cultures mostly make use of Bicarbonate – carbon dioxide buffering system to maintain pH.

When a transgene from a Genetically Modified crop escapes through pollen to a related plant species, it is known as:

Embryonic stem cells derived from the blastocyst stage of the embryo are ________________________ in nature.

An example of secondary metabolites produced by microbial cells include:

A protein ion with a molecular weight of 10,000 Daltons carried a charge of 5+ and was subjected to mass spectrometric analysis. Calculate its mass to charge ratio.

Identify the vector that infects E. coli cells containing F-plasmid and that has a single-stranded circular genome:}

Artificial seeds are produced by encapsulating the somatic embryos at the ______________ stage in a protective coating.

Severe combined immunodeficiency disease is caused due to the absence of:

Single nucleotide polymorphisms usually occur in ______________ regions.

The peptide hormones and growth factors to promote healthy growth of animal cells in vitro are often derived from:}

The natural source of enzyme barnase and barstar, a system used to achieve male sterile plant is:

Virus mediated transfer of host DNA from one cell to another cell is known as:

Match List I and List II

	List I: Fermentation Products		List II: Strain used
A	Mast cells	I	Clostridium tetani
B	Lymphocytes	II	Brevibacterium sp.
C	T-cells	III	Leuconostac mesenteroids
D	Monocytes- Macrophages	IV	Bacillus subtillis
		V	Streptomyces olivaceus

Choose the correct answer from the options given below:

Which of the following characteristics is most likely to be associated with a high apparent volume ofdistribution?

One of the following is correct match or mechanisms select it :