Question

All these statements are true regarding RFLP and RAPD except

• A. RAPD is quicker when compared to RFLP
• B. RFLP is more reliable than RAPD
• C. Species-specific primers are required for RAPD
• D. Radioactive probes are not used in RAPD

Hint:

RAPD uses random primers, whereas RFLP requires specific probes and is more reliable but slower.

Answer 1

The Correct Option is C

Step 1: Understanding RFLP and RAPD 
RFLP (Restriction Fragment Length Polymorphism) and RAPD (Random Amplified Polymorphic DNA) are two molecular techniques for detecting genetic diversity. 
Step 2: Evaluating the Options 
- RAPD is quicker when compared to RFLP: True, RAPD is quicker as it doesn’t require restriction enzyme digestion. 
- RFLP is more reliable than RAPD: True, RFLP is more specific and reliable for identifying polymorphisms. 
- Species-specific primers are required for RAPD: Incorrect, RAPD uses random primers, not species-specific ones. 
- Radioactive probes are not used in RAPD: True, RAPD typically uses non-radioactive detection methods. 
Step 3: Conclusion 
RAPD does not require species-specific primers.