List of top Biotechnology Questions

Telomerase functions as:
RNA primer at the 5’ ends of Okazaki fragments in prokaryotes are removed by:
Premature or incomplete protein synthesis happens due to:
The sequence of the structural genes in the lac operon is in the order of:
A double stranded DNA contains 42 percent of G and C. The percentage of ‘T’ is:
Which enzyme is involved in the base excision repair of DNA?
Which is true regarding a cosmid vector?
The mismatch repair system of E. coli is:
Lipogenesis is enhanced by:
Which is not true for karyotyping?
Which of the following methods can be used to detect single nucleotide change in DNA?
An improved strain of Penicillium, capable of producing higher concentration of antibiotic penicillin is:
Write the therapeutic use and the animal cell line employed in obtaining any three of the following protein pharmaceuticals:
A researcher performed protein fingerprinting on hemoglobin from both normal and sickled red blood cells. Complete the flow-chart of the process by filling A, B, and C.
A researcher performed protein fingerprinting
 
Discuss any three ways that can be employed to measure microbial cell growth.
Give the purification scheme for isolation of Humulin from E. coli.
Explain how the sterilization of the growth media is achieved in the laboratory.
Explain the reason for therapeutic use of whey proteins.
Discuss the development of a novel protein.
In Sanger's chain termination method, incorporation of ddNTP cause the growing DNA chains to terminate prematurely. Explain how.
(ii) Briefly write the steps of Sanger's chain termination method of DNA sequencing.
To perform DNA sequencing of a strand, we need to clone the sequence in a single-stranded form. Which vector will you prefer for this ?
Curd is advised to be administered with antibiotics. Why?
Name any two diseases that have been treated with whey.
Name any two properties that can be manipulated using Protein Engineering.
Why is single tube DNA sequencing considered better and safer ?