List of top Biotechnology Questions

In ELISA, which of the following enzymes are conjugated to antibodies for detection of the analyte?
P. Alkaline phosphatase
Q. Trypsinase
R. Horseradish peroxidase
S. Amylase
Which of the following methods are used for detection of DNA and RNA, respectively?
In hybridoma technology, which one of the following enzymes is absent in the myeloma cells that are used for monoclonal antibody production?

Viable cells (viability assay) are assayed by all of the following methods except 

Which one of the following is not a gene insertion technique into a eukaryotic cell
The total number of amino acids in human insulin (total number of amino acids in A and B chain) is
Pyrogens present in containers can be destroyed by heating the containers at:
During the denaturation cycle in polymerase chain reaction the temperature is raised to
Which of the following Microorganism is used for the effective synthesis of interferon?
Match the following enzymes in Column I with their respective functions under Column II
Column IColumn II
i. DNA ligase(p) Synthe size a DNA copy of RNA
ii. Alkaline phosphatase(q) Forms a bond between 3' -OH and 5'-PO4
iii. Reverse transcriptase(r) Removes terminal PO, from 3' or 5'end of DNA
iv. Polynucleotide kinase(s) Adds phosphate to 5'-OH end
When considering drug delivery to the brain which of the following is false?is short
Arrange the following steps in sequence of their order for production of recombinant Insulin:-
A. Fusion of A and B chains for disulphide bond.
B. Cynogen bromide treatment to remove methionine and â galactosidase.
C. Introduction of A and B chain in the plasmid containing â galactosidase gene.
D. Synthesis of A and B chain in E coil.
A technique of using very small metal particles coated with desired DNA in the gene transfer is called:-
Insulin and thyroxin arrive at an organ/tissue / cell at the same time. Thyroxine causes an effect on the organ but insulin does not because
A labeled piece of DNA that is complementary to the sequence of DNA you are interested in, say the gene you are trying to put into cells, is called as
Plasmodesmata is:
Match product, source and plant part form which they areobtained
(1)Bacosides(P)Aciacia catechu(i)Herb
(2)Cutch(Q)Rubiatictorium(ii)Leaf
(3)Henna(R)Bacopamonnieri(iii)Root
(4)Alizarm(S)Lawsoniainternis(iv)Stem
Which term describes 'The degree to which a set of inherent properties of a product system, of rocess fulfils requirements, the best'
Silicone based adhesive used in TDDS possess following properties:
Higuchi model is applicable to drug that is____In the formulation
Which micro-organism used in hairy root culture
The drug formulated as suspension follows...... order reaction
What are the three basic steps of conventional PCR
Identify the correct statement
(P) Condensed tannins are polymers flavans
(Q) Condensed tannins do not contain sugar redidues
(R) Hydrolyzabletannis are polymers of gallic acid or ellagic acids
(S) Gallic acid and catechin are psedotannins
Which RNA polymerase is the only whose product are capped