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questions
List of practice Questions
A 0.1 mL aliquot of a bacteriophage stock having a concentration of \(4 \times 10^9\) phages mL\(^{-1}\) is added to 0.5 mL of E. coli culture having \(2 \times 10^8\) cells mL\(^{-1}\). The multiplicity of infection is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
General Biology
Microbial diseases and host-pathogen interactions
If the area of a triangle with vertices \((k, 0)\), \((2, 0)\) and \((0, -2)\) is 2 square units, the value of \(k\) is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Engineering Mathematics
Solutions of linear and non-linear algebraic equations
In a chemostat with dilution rate 0.8 h\(^{-1}\), biomass concentration = 8 mol m\(^{-3}\), and specific product formation rate = 0.2 (mol product)(mol biomass)\(^{-1}\) h\(^{-1}\), the steady-state product concentration (mol m\(^{-3}\)) is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Bioprocess Engineering and Process Biotechnology
Batch, fed-batch and continuous processes
If the values of random variables \(X,Y\) are (121,360), (242,364), (363,362), the correlation coefficient (rounded to one decimal place) is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Engineering Mathematics
Regression and correlation analysis
It is desired to scale-up a fermentation from 1 L to 1000 L vessel by maintaining a constant power-to-volume ratio. The small fermenter is operated at an agitator speed of 300 rpm. If the value of scale-up factor is 10, agitator speed in rpm (rounded off to the nearest integer) for the large fermenter is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Bioprocess Engineering and Process Biotechnology
Optimization and scale up
The specific growth rate of a mold is 0.15 h\(^{-1}\). If the cell concentration at 30 h is 33 g L\(^{-1}\), the concentration at 24 h (rounded to nearest integer) is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Bioprocess Engineering and Process Biotechnology
Kinetics of cell growth
A sedimentation tank of height 100 cm is used to separate granular biomass of 0.5 mm diameter. Viscosity = 1 cP; density difference = 0.1 g cm\(^{-3}\). Settling time in min (rounded to two decimals) is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Bioprocess Engineering and Process Biotechnology
Filtration and Centrifugation
Tertiary structure of a protein consisting of $\alpha$-helices and $\beta$-strands can be determined by:
GATE BT - 2021
GATE BT
Biotechnology Tools and Techniques
Principles of spectroscopy
Which of the following statement(s) is/are CORRECT about
Agrobacterium tumefaciens
?
GATE BT - 2021
GATE BT
Plant, Animal and Microbial Biotechnology
Transgenic plants
Which of the following antimicrobial agent(s) is/are growth factor analog(s)?
GATE BT - 2021
GATE BT
General Biology
Antibiotics and antimicrobial resistance
Which of the following chemical messenger(s) is/are derivative(s) of tryptophan?
GATE BT - 2021
GATE BT
General Biology
Concepts and regulation of metabolism of amino acids
Which of the following nucleus/nuclei is/are NMR active?
GATE BT - 2021
GATE BT
Biotechnology Tools and Techniques
Principles of spectroscopy
In a Mendel's dihybrid experiment, a homozygous pea plant with round yellow seeds was crossed with a homozygous plant with wrinkled green seeds. F\(_1\) intercross produced 560 F\(_2\) progeny. The number of F\(_2\) progeny having both dominant traits (round and yellow) is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Genetics, Cellular and Molecular Biology
Principles of Mendelian inheritance
Which one of the following tools is used to compare all the possible six-open reading frames of a given nucleotide query sequence with all the available six-open reading frames of the nucleotide sequence database?
GATE BT - 2021
GATE BT
Biotechnology Tools and Techniques
Sequence analysis
In
Neurospora crassa
, a mutation in the
poky
gene results in slow growth (poky). The results of four crosses are given:
(1) wild-type ♀ × wild-type ♂ → All progeny wild-type
(2) wild-type ♀ × poky ♂ → All progeny wild-type
(3) poky ♀ × wild-type ♂ → All progeny poky
(4) poky ♀ × poky ♂ → All progeny poky
Which one of the following explains the inheritance mode of poky?
GATE BT - 2021
GATE BT
Genetics, Cellular and Molecular Biology
Extra chromosomal inheritance
The sum of the infinite geometric series \( 1 + \frac{1}{3} + \frac{1}{3^2} + \frac{1}{3^3} + \ldots \) (rounded off to one decimal place) is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Engineering Mathematics
Sequence and series
Three balls colored blue, green and red are transferred from box A to box B in the order BLUE–GREEN–RED. The probability that they are transferred back from box B to box A in the same order (rounded off to two decimal places) is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Engineering Mathematics
Random Variables
Decimal reduction time of a bacterial strain is 20 min. Specific death rate constant in min\(^{-1}\) (rounded off to two decimal places) is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Bioprocess Engineering and Process Biotechnology
Kinetics of cell growth
Two reactors of volumes 5 L and 10 L contain an ideal gas at 9 atm and 6 atm. When the valve is opened, the final equilibrium pressure (atm) of the system is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
Fundamentals of Biological Engineering
Reactive and non-reactive balances
For enzyme \(\alpha\)-amylase with \(K_m = 0.005\,M\), the substrate concentration for one-fourth of \(V_{\max}\) (rounded to two decimals, mM) is \(\underline{\hspace{1cm}}\).
GATE BT - 2021
GATE BT
General Biology
Enzyme kinetics: Michaelis-Menten equation
A protein without its prosthetic group is known as:
GATE BT - 2021
GATE BT
General Biology
Structure and function of biomolecules
The enzyme which adds phosphate group to the free 5' terminus of a DNA sequence is
GATE BT - 2021
GATE BT
Biotechnology Tools and Techniques
Restriction and modification enzymes
Which one of the following is CORRECT about microbial growth medium?
GATE BT - 2021
GATE BT
General Biology
Methods in microbiology
The cellular process which utilizes RNA-induced silencing complex to block gene expression is
GATE BT - 2021
GATE BT
Genetics, Cellular and Molecular Biology
RNA interference
Which of the following layer(s) is/are formed from the inner cell mass of the blastocyst?
GATE BT - 2021
GATE BT
Genetics, Cellular and Molecular Biology
Cell cycle and cell growth control
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