Question:

Which of the following is NOT correct about FASTA?

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FASTA is an early tool used for sequence alignment and similarity search. It uses "k-tuples" rather than blocks to identify short matching sequences in biological data.
Updated On: May 6, 2025
  • It was in fact the first database similarity search tool developed, preceding the development of BLAST
  • FASTA uses a ‘hashing’ strategy to find matches for a short stretch of identical residues with a length of k
  • The string of residues is known as blocks
  • It stands for FAST ALL
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The Correct Option is C

Solution and Explanation

Let's analyze each option:
(1) It was in fact the first database similarity search tool developed, preceding the development of BLAST: This is true. FASTA was an early database similarity search tool that preceded BLAST.
(2) FASTA uses a ‘hashing’ strategy to find matches for a short stretch of identical residues with a length of k: This is true. FASTA uses a hashing strategy to identify short matching sequences.
(3) The string of residues is known as blocks: This is untrue. The correct term used in FASTA is "k-tuples," not blocks. Hence, this is the incorrect statement and the correct answer.
(4) It stands for FAST ALL: This is true. FASTA stands for "Fast All," referring to the speed of the algorithm.
Conclusion: The correct answer is Option 3: The string of residues is known as blocks, as it is untrue. The correct term is "k-tuples."
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