Question

The melting temperature (Tm) of DNA is higher when the content of

• A. A: T base pair is lower
• B. G:C base pair is lower
• C. G:C base pair is higher
• D. A: T base pair is higher

Hint:

DNA with higher G:C content is more stable and has a higher melting temperature.

Answer 1

The Correct Option is C

The melting temperature (Tm) of DNA is determined by the strength of the bonds between base pairs. Since G:C base pairs form three hydrogen bonds, they are more stable and require more heat to separate than A:T base pairs, which only have two hydrogen bonds. Thus, a higher content of G:C base pairs leads to a higher melting temperature.
Conclusion: The melting temperature (Tm) of DNA is higher when the content of G:C base pairs is higher.