List of top Biochemistry Questions asked in GATE XL

Which one of the following double-stranded sequences will NOT be recognized by a Type IIP restriction endonuclease?

The spectrum of a protein obtained using electrospray ionization mass spectrometry (ESI-MS) is shown below. Two peaks, one at m/z = 2960.6 and the other at m/z = 3552.5, are marked. The mass of the protein associated with the m/z = 2960.6 peak is ______ Da. (Round off to two decimal places) 

A 5250 base-pair long plasmid with 10 negative supercoils would have a linking number of _______, considering 10.5 base pairs per turn for B DNA. (Answer in integer)
In Michaelis-Menten's equation, if \( [S] = 15 K_m \), then the ratio \( \frac{V_0}{V_{\text{max}}} \) is _______. (Round off to three decimal places)
Factor H, a complement regulatory protein in plasma, binds C3b and:
Which one or more of the following statements is/are correct in the processing of pre-mRNA in eukaryotes?
Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?
Which one or more of the following statements is/are correct regarding the transport and retention of proteins in different cell organelles?
Which one or more of the following statements is/are correct regarding the electromotive force generated by the electron transfer chain?
Which one of the following is the correct match between the molecular properties listed in Column I and the corresponding biochemical separation methods in Column II? \[ \begin{array}{|c|c|} \hline \textbf{Column I} & \textbf{Column II} \\ \hline P. \, \text{Solubility} & iii. \, \text{Salting out} \\ \hline Q. \, \text{Ionic charge} & iv. \, \text{Isoelectric focusing} \\ \hline R. \, \text{Polarity} & i. \, \text{Reverse phase chromatography} \\ \hline S. \, \text{Molecular size} & v. \, \text{Gel electrophoresis} \\ \hline \end{array} \]
Metabolic intermediates provide the backbone for the synthesis of amino acids. Match the metabolic intermediates listed in Column I with their corresponding amino acids given in Column II. \[ \begin{array}{|c|c|} \hline \textbf{Column I} & \textbf{Column II} \\ \hline P. \, (\alpha)-\text{Ketoglutarate} & ii. \, \text{Glutamate} \\ \hline Q. \, \text{Ribose 5-phosphate} & i. \, \text{Histidine} \\ \hline R. \, \text{3-Phosphoglycerate} & v. \, \text{Serine} \\ \hline S. \, \text{Phosphoenolpyruvate} & iii. \, \text{Aspartate} \\ \hline \end{array} \]
If a 10 mM solution of a biomolecule in a cuvette of path length 10 mm absorbs 90% of the incident light at 280 nm, the molar extinction coefficient of the biomolecule at this wavelength is _______ M(^{-1})cm(^{-1}). (Round off to two decimal places)
In a lactic acid solution at pH 4.8, the concentrations of lactic acid and lactate are 0.01 M and 0.087 M, respectively. The calculated pKa of lactic acid is _______. (Round off to one decimal place)
A newly identified viral protein contains one long α-helix spanning 60 amino acid residues. The number of main chain H-bonds formed in this helix is _______. (Answer in integer)
Which one or more of the following statements correctly describe(s) the addition of N-nucleotides during the rearrangement of the immunoglobulin heavy chain-encoding gene?
Which one or more of the following statements correctly describe(s) the changes upon the addition of puromycin during eukaryotic translation?
Mammalian cells cultured at low temperature (25 to 30 °C) lead to an increased sterol content in the membrane. Elevated sterols in the membrane result in
In mammals, hematopoietic stem cells that give rise to different types of blood cells are known as:
Which one of the following metabolic intermediates is common to glycolysis, nucleotide synthesis, and glycogen synthesis?
Which one of the following pairs of amino acids is NOT incorporated in a polypeptide chain?
Match the anti-nutritional factor in Column I with their corresponding activity given in Column II.
Column IColumn II
(P) Lectin 1 Total amount of produced by autotrophs energy
(Q) Stachyose  2Chelates with divalent cations and reduces their bio-availability 
(R) Phytate 3Inhibits trypsin and chymotrypsin 
(S) Knuitz type inhibitor 4Hemagglutination 
Which of the following fatty acids is/are known to increase the low density lipoprotein (LDL)-cholesterol?
Xeropthalmia is caused due to the deficiency of
Which one of the following statements is NOT TRUE of glycosaminoglycans?
When the blood glucose level of a healthy person is 100 mg/dL, which one of the following options is most likely to represent the level of glucose in the urine of that person?