Analysis of Each Statement: (A) It is used for the synthesis of ATP: This is correct. The electromotive force generated by the electron transfer chain is indeed used to drive the synthesis of ATP through chemiosmosis in the mitochondria.
(B) It is not used for active transport processes: This statement is incorrect. The proton gradient created by the electron transfer chain, part of the electromotive force, is used for various active transport processes across the mitochondrial membrane.
(C) It includes a pH gradient component: This is correct. The creation of a proton gradient, which involves a difference in pH across the mitochondrial membrane, is a critical component of the electromotive force used in ATP synthesis.
(D) It does not include an electrical potential gradient component: This statement is incorrect. The proton gradient results in both a pH gradient and an electrical potential gradient across the mitochondrial membrane. Conclusion:
Thus, the correct statements that accurately describe the electromotive force in the electron transfer chain are (A) and (C). This force is central to the mitochondrial process of energy conversion from nutrients to ATP, coupling electron transfer with proton translocation and ATP synthesis.
The spectrum of a protein obtained using electrospray ionization mass spectrometry (ESI-MS) is shown below. Two peaks, one at m/z = 2960.6 and the other at m/z = 3552.5, are marked. The mass of the protein associated with the m/z = 2960.6 peak is ______ Da. (Round off to two decimal places)