Question

Which one of the following is the correct match between the molecular properties listed in Column I and the corresponding biochemical separation methods in Column II? \[ \begin{array}{|c|c|} \hline \textbf{Column I} & \textbf{Column II} \\ \hline P. \, \text{Solubility} & iii. \, \text{Salting out} \\ \hline Q. \, \text{Ionic charge} & iv. \, \text{Isoelectric focusing} \\ \hline R. \, \text{Polarity} & i. \, \text{Reverse phase chromatography} \\ \hline S. \, \text{Molecular size} & v. \, \text{Gel electrophoresis} \\ \hline \end{array} \]

• A. ( P-i; Q-ii; R-v; S-iii )
• B. ( P-iii; Q-iv; R-ii; S-i )
• C. ( P-iii; Q-iv; R-i; S-ii )
• D. ( P-v; Q-iv; R-iii; S-ii )

Hint:

Understanding the principles behind each separation technique can greatly enhance your ability to design experiments and interpret results in biochemistry.

Answer 1

The Correct Option is C

P. Solubility is often manipulated through iii. Salting out, a method that alters solubility to precipitate proteins. 
Q. Ionic charge is the basis for iv. Isoelectric focusing, where proteins are separated based on their charge at different pH levels. 
R. Polarity influences interactions in i. Reverse phase chromatography, which separates molecules based on their hydrophobicity or hydrophilicity. S. Molecular size is a primary factor in ii. Ultracentrifugation, which separates particles based on size and density. Conclusion:
These matches highlight the specific properties that influence the separation techniques, providing a clear connection between molecular characteristics and biochemical methodologies.