List of top Biotechnology Questions asked in CBSE CLASS XII

What kind of information is available in UniProtKB and PDB databases?
Name the computer programmes that can perform gene prediction for bacterial and eukaryotic genomes.
How is BLAST used to analyse sequence similarity ? Explain.
Name three database retrieval tools available from the NCBI. What all do they allow us to access ?
During DNA sequencing, why is the autoradiogram read from bottom to top to arrive at the original sequence ?
In Sanger's chain termination method, incorporation of ddNTP cause the growing DNA chains to terminate prematurely. Explain how.
(ii) Briefly write the steps of Sanger's chain termination method of DNA sequencing.
Discuss the development of a novel protein.
Explain the reason for therapeutic use of whey proteins.
Explain how the sterilization of the growth media is achieved in the laboratory.
Name two phytohormones which are generally added to prepare plant nutrient media.
Which ingredient has been used to fulfill vitamin requirement?
Which component in the given list is acting as the carbon source?
Why is it advisable to use lesser number of steps for downstream processing?
Give the purification scheme for isolation of Humulin from E. coli.
Which step in the given purification scheme is metabolite specific?
Write whether Protein A is of intra or extra cellular origin.
Give the names of any three genes that are used as selectable markers in recombinant DNA technology. Also mention the trait/protein they specify.
What are zymogens? How is chymotrypsinogen different from chymotrypsin?
Discuss any three ways that can be employed to measure microbial cell growth.
A researcher performed protein fingerprinting on hemoglobin from both normal and sickled red blood cells. Complete the flow-chart of the process by filling A, B, and C.
A researcher performed protein fingerprinting
 
Explain the steps involved in PCR amplification method.
Write the therapeutic use and the animal cell line employed in obtaining any three of the following protein pharmaceuticals:
Compare the techniques of FISH with Microarray in terms of principle and applications.
Give any two drawbacks of animal cell culture in vitro.}
Write about any two strategies available to enhance the production of secondary metabolites in plant genetic engineering.