List of top Biotechnology Questions asked in CBSE Class Twelve Board Exam

An improved strain of Penicillium, capable of producing higher concentration of antibiotic penicillin is:
Write the therapeutic use and the animal cell line employed in obtaining any three of the following protein pharmaceuticals:
A researcher performed protein fingerprinting on hemoglobin from both normal and sickled red blood cells. Complete the flow-chart of the process by filling A, B, and C.
A researcher performed protein fingerprinting
 
Discuss any three ways that can be employed to measure microbial cell growth.
Give the purification scheme for isolation of Humulin from E. coli.
Explain how the sterilization of the growth media is achieved in the laboratory.
Explain the reason for therapeutic use of whey proteins.
Discuss the development of a novel protein.
In Sanger's chain termination method, incorporation of ddNTP cause the growing DNA chains to terminate prematurely. Explain how.
(ii) Briefly write the steps of Sanger's chain termination method of DNA sequencing.
Briefly write the steps of Sanger’s chain termination method of DNA sequencing.
Name any two diseases that have been treated with whey.
Curd is advised to be administered with antibiotics. Why?
Name any two properties that can be manipulated using Protein Engineering.
Why is single tube DNA sequencing considered better and safer ?
To perform DNA sequencing of a strand, we need to clone the sequence in a single-stranded form. Which vector will you prefer for this ?
During DNA sequencing, why is the autoradiogram read from bottom to top to arrive at the original sequence ?
Name three database retrieval tools available from the NCBI. What all do they allow us to access ?
Name the computer programmes that can perform gene prediction for bacterial and eukaryotic genomes.
What kind of information is available in UniProtKB and PDB databases?
How is BLAST used to analyse sequence similarity ? Explain.
Why is it advisable to use lesser number of steps for downstream processing?
Which ingredient has been used to fulfill vitamin requirement?
Name two phytohormones which are generally added to prepare plant nutrient media.
Which component in the given list is acting as the carbon source?
Explain the steps involved in PCR amplification method.