List of top Biotechnology Questions asked in CBSE CLASS XII

How is a continuous culture system maintained in microbial culture? What are its advantages over Fed-batch culture?
What is a “metagenome”? Describe the metagenomics approach and write its importance to study microorganisms.
Describe the procedure involved in Restriction Fragment Length Polymorphism (RFLP) technique. Why is this technique used in forensic sciences?
Describe the basic steps of Recombinant DNA Technology.
Mention any five protein-based products with an example of each.
Explain the technique of peptide mapping used to compare normal haemoglobin with sickle cell haemoglobin.
Write an application of callus culture.
What is meant by “Protoplast”?
Mention hormones that are used for promoting growth and cell division of plant cells in culture.
What is meant by explant culture?
Which enzyme is used in Sanger’s method of DNA sequencing?
What is the advantage of using ddNTPs conjugated with fluorescent molecules in automated method of DNA sequencing?
Write the difference between dNTPs and ddNTPs. What is the function of ddNTPs in Sanger’s method of DNA sequencing?
Why are primers required in Sanger’s method of DNA sequencing?
A comparative microarray hybridization experiment was performed between normal and cancerous cells. How will you interpret the result obtained on a microarray, if red and green coloured fluors were used for labelling the cDNA probes of normal and cancerous cells respectively?
Transgenic plants can be used as factories to produce polyhydroxybutyrate (PHB) on a large scale. How was Arabidopsis plant engineered for the same? State the drawback of producing PHB by fermentation using bacterium Alcaligenes eutrophus.
It is very difficult to produce hybrids in case of interspecific and intergeneric crosses. Why? Briefly describe the technique to obtain such novel hybrids.
Although not required for cell growth, antibiotics are added in animal cell culture medium. Give reason. Name two such antibiotics.
What is the mode of action of tissue Plasminogen Activator (tPA)? Draw a schematic representation to show the method of production of tPA through mammalian cell culture.
Explain the method of Blue-White selection used for screening of recombinant cells containing desired plasmid with gene of interest.
Mention the important features that were incorporated in each of the following vectors:
(i) COSMIDS
(ii) Shuttle Vectors
(iii) Expression Vectors
Elaborate upon the scientific relevance for therapeutic usefulness of whey with specific examples.
What is the most common cause of foaming in microbial culture medium? Which problems are created by foaming in microbiological processes?
Outline the important steps for isolation of recombinant insulin (Humulin) from Escherichia coli.
Why is chronic myelogenous leukemia caused? Name the technique used to know the status of this disease.