Question

Give the names of any three genes that are used as selectable markers in recombinant DNA technology. Also mention the trait/protein they specify.

Hint:

Remember that selectable marker genes are useful tools in recombinant technology, and try to know the particular trait/protein associated with them.

Answer 1

Ampicillin resistance gene: This gene encodes a protein that makes the bacteria resistant to the antibiotic ampicillin, and hence gives the bacteria a selective advantage in the presence of ampicillin. Lac Z gene: The Lac Z gene encodes an enzyme called beta-galactosidase which is required for breaking down the disaccharide lactose. GFP gene: The GFP (Green Fluorescent Protein) gene encodes a protein that fluoresces green under UV light. Tetracycline resistance gene: This provides resistance against the antibiotic tetracycline. Leu 2 gene: This is a marker gene which codes for an enzyme needed for synthesis of an amino acid, leucine.