List of top Biotechnology Questions asked in CBSE Class Twelve Board Exam

Why is chronic myelogenous leukemia caused? Name the technique used to know the status of this disease.

Outline the important steps for isolation of recombinant insulin (Humulin) from Escherichia coli.

What is the most common cause of foaming in microbial culture medium? Which problems are created by foaming in microbiological processes?

_______ can be categorized as a food source of nutraceutical proteins.

Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): Proteins have diverse functions.
Reason (R): All proteins are enzymes.

Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): One of the first attempts to study the molecular basis of sickle cell anaemia was to compare the electrophoretic mobility of normal haemoglobin (Hb) and sickle cell haemoglobin (scHb).
Reason (R): Sickle cell haemoglobin (scHb) moved faster than normal haemoglobin (Hb) in electrophoresis.

Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): CO₂ incubators prevent the desiccation of the animal cell culture medium.
Reason (R): A pan of water is kept at all times in a CO₂ incubator chamber to maintain high relative humidity.

Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): Contact inhibition is absent in cancer cells.
Reason (R): Normal cells stop growing when they reach the walls of the container.

Identification of disease specific proteins can be done by using the approach of:

Herceptin is a monoclonal antibody approved for:

One of the carbon sources used in microbial cell culture is:

The technique to isolate and grow human embryonic stem cells in culture was developed by:

Gene prediction for bacterial genomes can be done by using the computer program _______.

Single base difference in ApoE gene is associated with:

Absence of the enzyme adenosine deaminase in humans causes:

A biotechnologist wants to obtain a gene sequence in single stranded form. Which bacteriophage based vector should he choose to obtain the desired result?

Protein Efficiency Ratio (PER) is used as a measure of growth expressed in terms of:

At the end of one cycle of PCR, two DNA molecules will become ________ .

The DNA ligase enzyme that is frequently used to ligate different DNA fragments in order to generate rDNA molecules is isolated from:

An improved strain of Penicillium, capable of producing higher concentration of antibiotic penicillin is:

Write the therapeutic use and the animal cell line employed in obtaining any three of the following protein pharmaceuticals:

A researcher performed protein fingerprinting on hemoglobin from both normal and sickled red blood cells. Complete the flow-chart of the process by filling A, B, and C.

 

Discuss any three ways that can be employed to measure microbial cell growth.

Give the purification scheme for isolation of Humulin from E. coli.

Explain how the sterilization of the growth media is achieved in the laboratory.