Overexpression of Genes: One strategy is to overexpress the genes that encode for the first enzyme in a biosynthetic pathway. The first enzyme in the pathway is often the rate limiting step, and increasing its activity will increase the production of the end product.
Agrobacterium-mediated induction of secondary roots: Another technique is to use Agrobacterium rhizogenes} to induce the formation of excessive secondary roots in plants that normally produce useful secondary metabolites in these roots, increasing their production and their harvesting.