Question:
To perform DNA sequencing of a strand, we need to clone the sequence in a single-stranded form. Which vector will you prefer for this ?
To perform DNA sequencing of a strand, we need to clone the sequence in a single-stranded form. Which vector will you prefer for this ?
Updated On: June 02, 2025
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Solution and Explanation
Vector Preference for Single-Stranded DNA Sequencing: M13-based vector will
be the preferred vector for sequencing single stranded DNA. This vector carries single
stranded DNA within the viral particles, which makes it ideal for cloning
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