Question

A biotechnologist wants to obtain a gene sequence in single stranded form. Which bacteriophage based vector should he choose to obtain the desired result?

• A. M13 based vector
• B. Bacteriophage Lambda based vector
• C. Plasmid cloning vector of pUC family
• D. Plasmid vector pBR 322

Hint:

M13 vectors are preferred for producing single-stranded DNA, useful in sequencing and mutagenesis.

Answer 1

The Correct Option is A

M13 bacteriophage based vectors are filamentous phages used in molecular cloning to produce single-stranded DNA. This property makes them ideal for applications like DNA sequencing or site-directed mutagenesis where single-stranded DNA is required.

• M13 based vector: Produces single-stranded DNA, suitable for gene sequencing.
• Lambda bacteriophage vector: Typically produces double-stranded DNA.
• Plasmid vectors (pUC family, pBR 322): Circular double-stranded DNA vectors used for cloning but do not generate single-stranded DNA naturally.

Hence, the correct choice is (A) M13 based vector.