Question

Which enzyme is used in Sanger’s method of DNA sequencing?

Hint:

DNA polymerase extends the primer by adding nucleotides and is essential for DNA synthesis in Sanger sequencing.

Answer 1

The enzyme used in Sanger’s method of DNA sequencing is DNA polymerase. This enzyme synthesizes a new DNA strand by adding nucleotides complementary to the template strand. It incorporates both normal deoxynucleotides (dNTPs) and chain-terminating dideoxynucleotides (ddNTPs) during DNA synthesis, allowing sequence determination based on chain termination.