List of top Biotechnology Questions

What is the mode of action of tissue Plasminogen Activator (tPA)? Draw a schematic representation to show the method of production of tPA through mammalian cell culture.
Explain the method of Blue-White selection used for screening of recombinant cells containing desired plasmid with gene of interest.
Mention the important features that were incorporated in each of the following vectors:
(i) COSMIDS
(ii) Shuttle Vectors
(iii) Expression Vectors
Elaborate upon the scientific relevance for therapeutic usefulness of whey with specific examples.
What is the most common cause of foaming in microbial culture medium? Which problems are created by foaming in microbiological processes?
Outline the important steps for isolation of recombinant insulin (Humulin) from Escherichia coli.
Why is chronic myelogenous leukemia caused? Name the technique used to know the status of this disease.
With a suitable example, explain briefly, why the number of predicted genes do not correlate with the genome size and the number of chromosomes in an organism.
Describe briefly, the charge relay system that operates in chymotrypsin enzyme.
What is the principle of mass spectrometry? Write its important application.
Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): One of the first attempts to study the molecular basis of sickle cell anaemia was to compare the electrophoretic mobility of normal haemoglobin (Hb) and sickle cell haemoglobin (scHb).
Reason (R): Sickle cell haemoglobin (scHb) moved faster than normal haemoglobin (Hb) in electrophoresis.
Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): Proteins have diverse functions.
Reason (R): All proteins are enzymes.
Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): Contact inhibition is absent in cancer cells.
Reason (R): Normal cells stop growing when they reach the walls of the container.
Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): CO2 incubators prevent the desiccation of the animal cell culture medium.
Reason (R): A pan of water is kept at all times in a CO2 incubator chamber to maintain high relative humidity.
Herceptin is a monoclonal antibody approved for:
Identification of disease specific proteins can be done by using the approach of:
_______ can be categorized as a food source of nutraceutical proteins.
Gene prediction for bacterial genomes can be done by using the computer program _______.
The technique to isolate and grow human embryonic stem cells in culture was developed by:
Absence of the enzyme adenosine deaminase in humans causes:
A biotechnologist wants to obtain a gene sequence in single stranded form. Which bacteriophage based vector should he choose to obtain the desired result?
One of the carbon sources used in microbial cell culture is:
Single base difference in ApoE gene is associated with:
At the end of one cycle of PCR, two DNA molecules will become ________ .
Protein Efficiency Ratio (PER) is used as a measure of growth expressed in terms of: