List of top Biotechnology Questions

Describe briefly, the charge relay system that operates in chymotrypsin enzyme.

Outline the important steps for isolation of recombinant insulin (Humulin) from Escherichia coli.

What is the most common cause of foaming in microbial culture medium? Which problems are created by foaming in microbiological processes?

Elaborate upon the scientific relevance for therapeutic usefulness of whey with specific examples.

Herceptin is a monoclonal antibody approved for:

Identification of disease specific proteins can be done by using the approach of:

Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): CO₂ incubators prevent the desiccation of the animal cell culture medium.
Reason (R): A pan of water is kept at all times in a CO₂ incubator chamber to maintain high relative humidity.

Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): Contact inhibition is absent in cancer cells.
Reason (R): Normal cells stop growing when they reach the walls of the container.

Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): One of the first attempts to study the molecular basis of sickle cell anaemia was to compare the electrophoretic mobility of normal haemoglobin (Hb) and sickle cell haemoglobin (scHb).
Reason (R): Sickle cell haemoglobin (scHb) moved faster than normal haemoglobin (Hb) in electrophoresis.

_______ can be categorized as a food source of nutraceutical proteins.

Two statements are given – one labelled Assertion (A) and the other labelled Reason (R). Select the correct answer from the codes given below:
Assertion (A): Proteins have diverse functions.
Reason (R): All proteins are enzymes.

A biotechnologist wants to obtain a gene sequence in single stranded form. Which bacteriophage based vector should he choose to obtain the desired result?

Single base difference in ApoE gene is associated with:

Absence of the enzyme adenosine deaminase in humans causes:

One of the carbon sources used in microbial cell culture is:

The technique to isolate and grow human embryonic stem cells in culture was developed by:

Gene prediction for bacterial genomes can be done by using the computer program _______.

The DNA ligase enzyme that is frequently used to ligate different DNA fragments in order to generate rDNA molecules is isolated from:

At the end of one cycle of PCR, two DNA molecules will become ________ .

Protein Efficiency Ratio (PER) is used as a measure of growth expressed in terms of:

The overall stoichiometry for aerobic cell growth is:
3C₆H₁₂O₆ + 2.5NH₃ + O₂ → 1.5C₆H₈O₄Na + 3CO₂ + 5H₂O

The elemental composition formula of the biomass is: