List of top Biotechnology Questions

What kind of information is available in UniProtKB and PDB databases?

Name three database retrieval tools available from the NCBI. What all do they allow us to access ?

During DNA sequencing, why is the autoradiogram read from bottom to top to arrive at the original sequence ?

How is BLAST used to analyse sequence similarity ? Explain.

Name the computer programmes that can perform gene prediction for bacterial and eukaryotic genomes.

Which component in the given list is acting as the carbon source?

Why is it advisable to use lesser number of steps for downstream processing?

Which ingredient has been used to fulfill vitamin requirement?

Name two phytohormones which are generally added to prepare plant nutrient media.

What are zymogens? How is chymotrypsinogen different from chymotrypsin?

Which step in the given purification scheme is metabolite specific?

Write whether Protein A is of intra or extra cellular origin.

Give the names of any three genes that are used as selectable markers in recombinant DNA technology. Also mention the trait/protein they specify.

Explain the steps involved in PCR amplification method.

Write about any two strategies available to enhance the production of secondary metabolites in plant genetic engineering.

Compare the techniques of FISH with Microarray in terms of principle and applications.

What are the advantages offered by creating a mouse model with gene knockout?

Name the technique that helps to study the entire protein profile from a given cell type. Briefly explain the principle of this method.

Illustrate steps to show the construction of a recombinant DNA molecule.

Give any two drawbacks of animal cell culture in vitro.}

Assertion (A): Some experts believe that there must be more than 30,000 genes in human genome.
Reason (R): Unreliability of in silico gene prediction is responsible for reporting lesser number of genes in human genome.

________________________ cultures can be maintained for a prolonged period of time by repeated sub-culturing.

Assertion (A): The regulation of pH is essential for survival of mammalian cells.
Reason (R): Animal cell cultures mostly make use of Bicarbonate – carbon dioxide buffering system to maintain pH.

Assertion (A): The exact chemical composition of complex microbial growth media is known.
Reason (R): Complex nutrient media is used when specific growth requirement of a microorganism is unknown.

Assertion (A): During plant tissue culture, the explants are treated with sodium hypochlorite.
Reason (R): Sodium hypochlorite helps in acclimatization of the regenerated plants.