List of top Biotechnology Questions

An electric circuit with a resistor of constant resistance ‘R’ is maintained at a constant voltage ‘V’. Based on Ohm’s law, if the current ‘I’ through the circuit is doubled, the power ‘P’ dissipated across the resistor is
The correct order of electronegativity of the given elements is
What is the ploidy level of a sporophyte in angiosperms?
Which of the following terrestrial biomes has the highest animal diversity?
After how many cycles of polymerase chain reaction (PCR), the amplified product of required length is generated for the first time from a chromosomal DNA template?
Which one of the following hormones is produced by the adrenal gland?
In humans, Down syndrome is caused by
Which strategy can be used to minimize the shear damage in bioreactors used for animal cell culture?
How is a continuous culture system maintained in microbial culture? What are its advantages over Fed-batch culture?
Write the difference between dNTPs and ddNTPs. What is the function of ddNTPs in Sanger’s method of DNA sequencing?
Describe the procedure involved in Restriction Fragment Length Polymorphism (RFLP) technique. Why is this technique used in forensic sciences?
What is a “metagenome”? Describe the metagenomics approach and write its importance to study microorganisms.
Describe the basic steps of Recombinant DNA Technology.
Mention hormones that are used for promoting growth and cell division of plant cells in culture.
What is meant by explant culture?
Explain the technique of peptide mapping used to compare normal haemoglobin with sickle cell haemoglobin.
What is meant by “Protoplast”?
Mention any five protein-based products with an example of each.
Write an application of callus culture.
What is the advantage of using ddNTPs conjugated with fluorescent molecules in automated method of DNA sequencing?
Which enzyme is used in Sanger’s method of DNA sequencing?
What is the mode of action of tissue Plasminogen Activator (tPA)? Draw a schematic representation to show the method of production of tPA through mammalian cell culture.
It is very difficult to produce hybrids in case of interspecific and intergeneric crosses. Why? Briefly describe the technique to obtain such novel hybrids.
Explain the method of Blue-White selection used for screening of recombinant cells containing desired plasmid with gene of interest.
A comparative microarray hybridization experiment was performed between normal and cancerous cells. How will you interpret the result obtained on a microarray, if red and green coloured fluors were used for labelling the cDNA probes of normal and cancerous cells respectively?