List of top Biotechnology Questions

In Sanger's chain termination method, incorporation of ddNTP cause the growing DNA chains to terminate prematurely. Explain how.
(ii) Briefly write the steps of Sanger's chain termination method of DNA sequencing.
Discuss the development of a novel protein.
Explain the reason for therapeutic use of whey proteins.
Explain how the sterilization of the growth media is achieved in the laboratory.
Name two phytohormones which are generally added to prepare plant nutrient media.
Which ingredient has been used to fulfill vitamin requirement?
Which component in the given list is acting as the carbon source?
Why is it advisable to use lesser number of steps for downstream processing?
Give the purification scheme for isolation of Humulin from E. coli.
Which step in the given purification scheme is metabolite specific?
Write whether Protein A is of intra or extra cellular origin.
Give the names of any three genes that are used as selectable markers in recombinant DNA technology. Also mention the trait/protein they specify.
What are zymogens? How is chymotrypsinogen different from chymotrypsin?
Discuss any three ways that can be employed to measure microbial cell growth.
A researcher performed protein fingerprinting on hemoglobin from both normal and sickled red blood cells. Complete the flow-chart of the process by filling A, B, and C.
A researcher performed protein fingerprinting
 
Explain the steps involved in PCR amplification method.
Write the therapeutic use and the animal cell line employed in obtaining any three of the following protein pharmaceuticals:
Compare the techniques of FISH with Microarray in terms of principle and applications.
Give any two drawbacks of animal cell culture in vitro.}
Write about any two strategies available to enhance the production of secondary metabolites in plant genetic engineering.
Name the technique that helps to study the entire protein profile from a given cell type. Briefly explain the principle of this method.
What are the advantages offered by creating a mouse model with gene knockout?
Illustrate steps to show the construction of a recombinant DNA molecule.
Assertion (A): During plant tissue culture, the explants are treated with sodium hypochlorite.
Reason (R): Sodium hypochlorite helps in acclimatization of the regenerated plants.
Assertion (A): The regulation of pH is essential for survival of mammalian cells.
Reason (R): Animal cell cultures mostly make use of Bicarbonate – carbon dioxide buffering system to maintain pH.