List of top Biotechnology Questions

Give the names of any three genes that are used as selectable markers in recombinant DNA technology. Also mention the trait/protein they specify.

Write about any two strategies available to enhance the production of secondary metabolites in plant genetic engineering.

What are the advantages offered by creating a mouse model with gene knockout?

Compare the techniques of FISH with Microarray in terms of principle and applications.

Give any two drawbacks of animal cell culture in vitro.}

Name the technique that helps to study the entire protein profile from a given cell type. Briefly explain the principle of this method.

Illustrate steps to show the construction of a recombinant DNA molecule.

Assertion (A): During plant tissue culture, the explants are treated with sodium hypochlorite.
Reason (R): Sodium hypochlorite helps in acclimatization of the regenerated plants.

Assertion (A): Some experts believe that there must be more than 30,000 genes in human genome.
Reason (R): Unreliability of in silico gene prediction is responsible for reporting lesser number of genes in human genome.

________________________ cultures can be maintained for a prolonged period of time by repeated sub-culturing.

Assertion (A): The regulation of pH is essential for survival of mammalian cells.
Reason (R): Animal cell cultures mostly make use of Bicarbonate – carbon dioxide buffering system to maintain pH.

Assertion (A): The exact chemical composition of complex microbial growth media is known.
Reason (R): Complex nutrient media is used when specific growth requirement of a microorganism is unknown.

When a transgene from a Genetically Modified crop escapes through pollen to a related plant species, it is known as:

Embryonic stem cells derived from the blastocyst stage of the embryo are ________________________ in nature.

An example of secondary metabolites produced by microbial cells include:

A protein ion with a molecular weight of 10,000 Daltons carried a charge of 5+ and was subjected to mass spectrometric analysis. Calculate its mass to charge ratio.

Artificial seeds are produced by encapsulating the somatic embryos at the ______________ stage in a protective coating.

Severe combined immunodeficiency disease is caused due to the absence of:

Single nucleotide polymorphisms usually occur in ______________ regions.

The peptide hormones and growth factors to promote healthy growth of animal cells in vitro are often derived from:}

Identify the vector that infects E. coli cells containing F-plasmid and that has a single-stranded circular genome:}

The natural source of enzyme barnase and barstar, a system used to achieve male sterile plant is: