Question

Name the technique that helps to study the entire protein profile from a given cell type. Briefly explain the principle of this method.

Hint:

Be clear about the principle and purpose of proteomics techniques like Two Dimensional Gel Electrophoresis and Mass Spectrometry.

Answer 1

Technique: Two-Dimensional Gel Electrophoresis Technique combined with Mass Spectrometry or Mass Spectrometry Technique can be used for analyzing the complete protein profile of a cell. Principle of Two-dimensional Gel Electrophoresis Technique: This method separates proteins based on their charge and size. In the first dimension, the proteins are separated using Isoelectric focusing (IEF) based on their isoelectric point. Then, the proteins are separated based on their molecular weight using SDS PAGE (sodium dodecyl-sulfate polyacrylamide gel electrophoresis). Principle of Mass Spectrometry Technique: This technique determines the molecular weight of a chemical compound or protein by separating the molecular ions according to their mass-to-charge (m/z) ratio.