Question

The method(s) used to detect a DNA fragment of 150 base pairs is/are

• A. agarose gel electrophoresis
• B. northern blotting
• C. polyacrylamide gel electrophoresis
• D. western blotting

Hint:

For DNA detection, use agarose gel electrophoresis. Northern blotting and western blotting are for RNA and protein, respectively.

Answer 1

The Correct Option is A

Step 1: Understanding the methods.
- Agarose gel electrophoresis is the most commonly used method to separate and visualize DNA fragments. It allows DNA of various sizes to be separated by size, with shorter fragments traveling faster than longer ones. A DNA fragment of 150 base pairs can be easily separated using this method.
- Northern blotting involves the detection of RNA, not DNA, and thus is not suitable for detecting a DNA fragment.
- Polyacrylamide gel electrophoresis is typically used for separating smaller fragments, such as proteins or smaller nucleic acid sequences, but for 150 base pairs, agarose gel electrophoresis is more suitable.
- Western blotting is used to detect proteins, not nucleic acids, so it is not applicable for detecting DNA fragments.
Step 2: Conclusion.
The correct method to detect a DNA fragment of 150 base pairs is agarose gel electrophoresis.
Final Answer: \[ \boxed{\text{Agarose gel electrophoresis is used to detect a DNA fragment of 150 base pairs.}} \]