Question

Which of the following restriction enzymes produces blunt ends?

• A. HindIII
• B. SalI
• C. EcoRV
• D. XhoI

Hint:

Restriction enzymes that cut DNA without leaving overhangs (blunt ends) are useful for certain cloning applications.

Answer 1

The Correct Option is C

Step 1: Understanding blunt ends.
Blunt ends are formed when both strands of the DNA are cut at the same position, without any overhang.
Some restriction enzymes cut DNA to produce blunt ends.
Step 2: Identifying EcoRV.
EcoRV is a restriction enzyme that cuts DNA to produce blunt ends.
It cleaves the DNA at specific recognition sites without leaving overhanging bases.
Step 3: Reviewing other options.
HindIII: This enzyme cuts to produce sticky ends with overhangs.
SalI: This enzyme also creates sticky ends, not blunt ends.
XhoI: This is another enzyme that creates sticky ends.

Thus, the correct answer is (C) EcoRV.