Question

The correct order of steps in the process of recombinant DNA technology are:
1. Digestion by restriction enzyme
2. Transformation
3. Ligation
4. Isolation of DNA

• A. (D), (C), (B), (A)
• B. (A), (C), (B), (D)
• C. (D), (A), (C), (B)
• D. (A), (D), (C), (B)

Hint:

Recombinant DNA technology requires precise execution of steps in the correct order to achieve the desired genetic modification.

Answer 1

The Correct Option is C

The steps in recombinant DNA technology occur in the following order:
1. Isolation of DNA (D): The DNA is isolated from the source organism.
2. Digestion by restriction enzyme (A): Specific restriction enzymes are used to cut the DNA at desired sites.
3. Ligation (C): The DNA fragments are joined together using DNA ligase.
4. Transformation (B): The recombinant DNA is introduced into a host organism for replication and expression.
This sequence ensures that the recombinant DNA is properly prepared and successfully introduced into the host.