Question

Study the diagram given below that shows the steps involved in the procedure of selecting transformed bacteria and answer the questions that follow: 

 (a) Identify the colony that has got transformed. Justify your answer. 
 

Hint:

In transformation experiments: - Disrupted resistance gene = Loss of resistance. - Growth indicates retained resistance (e.g., ampicillin resistance).

Answer 1

- The transformed colony is the one that grows on Plate M (with ampicillin) but does not grow on Plate N (with both ampicillin and tetracycline). - Justification: The recombinant DNA disrupts the tetracycline resistance gene, making the bacteria sensitive to tetracycline, but retains ampicillin resistance, allowing growth on Plate M.