List of practice Questions

Give the purification scheme for isolation of Humulin from E. coli.

Explain how the sterilization of the growth media is achieved in the laboratory.

Explain the reason for therapeutic use of whey proteins.

Discuss the development of a novel protein.

In Sanger's chain termination method, incorporation of ddNTP cause the growing DNA chains to terminate prematurely. Explain how.
(ii) Briefly write the steps of Sanger's chain termination method of DNA sequencing.

Why is single tube DNA sequencing considered better and safer ?

To perform DNA sequencing of a strand, we need to clone the sequence in a single-stranded form. Which vector will you prefer for this ?

Briefly write the steps of Sanger’s chain termination method of DNA sequencing.

Name any two properties that can be manipulated using Protein Engineering.

Name any two diseases that have been treated with whey.

Curd is advised to be administered with antibiotics. Why?

During DNA sequencing, why is the autoradiogram read from bottom to top to arrive at the original sequence ?

Name three database retrieval tools available from the NCBI. What all do they allow us to access ?

How is BLAST used to analyse sequence similarity ? Explain.

Name the computer programmes that can perform gene prediction for bacterial and eukaryotic genomes.

What kind of information is available in UniProtKB and PDB databases?

Why is it advisable to use lesser number of steps for downstream processing?

Which component in the given list is acting as the carbon source?

Which ingredient has been used to fulfill vitamin requirement?

Name two phytohormones which are generally added to prepare plant nutrient media.

Explain the steps involved in PCR amplification method.

What are zymogens? How is chymotrypsinogen different from chymotrypsin?

Give the names of any three genes that are used as selectable markers in recombinant DNA technology. Also mention the trait/protein they specify.

Write whether Protein A is of intra or extra cellular origin.

Which step in the given purification scheme is metabolite specific?