List of top Questions asked in Indian Institute Of Technology Joint Admission Test for MSc

In the ‘Southern blot’ technique, which of the following reagents is used to detect the presence of a desired DNA fragment?

$K_m$ and $V_{max}$ of an enzyme preparation are 5 µM and 30 $µM min^{-1}$ respectively. Considering, $K_i$ value of competitive inhibitor is 60 µM, the velocity ($V_0$) of this enzyme-catalyzed reaction in the presence of 200 µM of substrate and 600 µM of competitive inhibitor is _______ $ µM min^{-1}$ (rounded off to two decimal places).

A genetic linkage map represents the

Class II MHC molecules are NOT expressed by

The value of $\lim_{𝑥→−3}\frac{(2𝑥+6)}{(𝑥+3)}$ is

Match the microorganisms in Group I with the human disease in Group II

Group I	Group II
P) Treponema pallidum	1) Sleeping sickness
Q) Trypanosoma cruzi	2) Whooping cough
R) Trypanosoma gambiense	3) Chagas disease
S) Bordetella pertussis	4) Syphilis

A growing shoot of a germinating seedling encounters an underground obstacle. Which one of the following hormones elicits ‘triple response’ to the underground obstacle?

Match the molecules in Group I with the type of bonds present in them, in Group II

Group I	Group II
P) NaCl	1) Coordination bond
Q) $H_2$	2) Polar covalent bond
R) $Pd-P$ bond in $Pd(PPh_3)_4	3) Covalent bond
S) $C-Cl$ bond in $CH_3Cl $	4) Ionic bond

Match the recombinant DNA products in Group I with its application in Group II

Group I	Group II
P) Tissue plasminogen activator	1) Emergency treatment of heart attack
Q) Erythropoietin	2) Treatment of anemia
R) Superoxide dismutase	3) Prevents tissue damage
S) Interferon	4) Stimulates cells to inhibit viral replication

A species of fish living in a lake are separated by drying up of the lake into two separate lakes. After several hundreds of years of separation, the two groups are unable to mate. These groups are now considered to be different _____.

Match the type of DNA repair mechanism in Group I with the enzyme(s) involved in Group II

Group I	Group II
P) Mismatch repair	1) DNA glycosylase
Q) Base excision repair	2) UvrA, UvrB, UvrC and UvrD
R) Nucleotide excision repair	3) RecA
S) Double strand break repair	4) MutL, MutS and MutH

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion [a]: Nitric oxide is involved in transient paracrine and autocrine signaling.
Reason [r]: Nitric oxide is highly reactive, with a lifetime of few seconds, yet can diffuse freely across membranes

Rain is falling vertically with a speed of 40 $m s^{-1}$. Wind starts blowing with a speed of 16 $m s^{-1}$ in the west to east direction. How should a person, who is standing, hold his umbrella to avoid getting wet?

Which of the following statement(s) is/are correct about telophase?

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion [a]: The cardiovascular organization called double circulation provides vigorous flow of blood to the brain, muscles, and other organs.
Reason [r]: The blood is pumped a second time after it loses pressure in the capillary beds of the lungs or skin.

Restriction enzymes that recognize the same nucleotide sequence but cleave at different positions are called

Tetracycline binds to the

Different segments of a photosynthetic filamentous alga are exposed to different wavelengths of light as shown below. After a period of time, bacteria known to migrate towards high oxygen concentration, is spread on the surface of the alga. Which region(s) of the alga will have maximum bacterial congregation?

The characteristic morphological change(s) in cells undergoing apoptosis is/are

The pedigree given below shows individuals affected (shaded circles/rectangles) by chronic hypertension. Assuming 100% penetrance, the inheritance of this trait is

The order of differential equation $\frac{𝑑^3𝑦}{dx^3} + 2 \frac{𝑑^2𝑦}{dx^2} − 3 \frac{𝑑𝑦}{dx} + 6𝑥^4𝑦$ = 0 is _______.

The number of possible unique combination(s) of linear tetrapeptides that can be made from four different amino acids using each amino acid only once in the chain is/are _____.

Given data consists of distinct values of $𝑥_𝑖$ occurring with frequencies $𝑓_𝑖.$ The mean value for the data is _____. (rounded off to one decimal place)

$X_i$	5	6	8	10
$F_i$	8	10	10	12

The net number of molecule(s) of NADH formed from one molecule of glucose in glycolysis under aerobic conditions is/are _______.

Among $K^+, Li^+, Rb^+, Cs^+$, the number of cation(s) having ionic radii more than $Na^+$ is/are _____.