Question

Which one of the following tools of recombinant DNA technology is INCORRECTLY paired with its applications?

• A. restriction endonuclease - production of DNA fragments for gene cloning.
• B. DNA ligase - enzyme that cuts DNA, creating sticky ends
• C. DNA polymerase - copies DNA sequences in the polymerase chain reaction
• D. reverse transcriptase - production of cDNA from mRNA

Hint:

When working with recombinant DNA technology, remember that DNA ligase joins fragments, while restriction enzymes cut DNA to produce sticky ends.

Answer 1

The Correct Option is B

DNA ligase is used to join DNA fragments together by forming covalent bonds between nucleotides. It does not cut DNA to create sticky ends. Instead, restriction endonucleases are responsible for cutting DNA and creating sticky ends.