Step 1: Understanding the role of linkage. - Linkage refers to the inheritance of genes located close together on the same chromosome. - Since linked genes are inherited together, they do not contribute to genetic variation.
Step 2: Contrasting linkage with other mechanisms. - Independent assortment, crossing over, and mutations result in genetic diversity by introducing new combinations or changes in genetic material. - Linkage conserves the gene arrangement, thereby reducing variation.
In the above represented plasmid an alien piece of DNA is inserted at the EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?