Reverse transcriptase is an enzyme that synthesizes complementary DNA (cDNA) from an RNA template. This process is a key step in the life cycle of retroviruses like HIV.

In the above represented plasmid an alien piece of DNA is inserted at the EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
Match Fibre with Application.\[\begin{array}{|l|l|} \hline \textbf{LIST I} & \textbf{LIST II} \\ \textbf{Fibre} & \textbf{Application} \\ \hline \hline \text{A. Silk fibre} & \text{I. Fire retardant} \\ \hline \text{B. Wool fibre} & \text{II. Directional lustre} \\ \hline \text{C. Nomex fibre} & \text{III. Bulletproof} \\ \hline \text{D. Kevlar fibre} & \text{IV. Thermal insulation} \\ \hline \end{array}\]