Question

Which strategy can be used to minimize the shear damage in bioreactors used for animal cell culture?

• A. Using spargers
• B. Increasing agitation rate
• C. Adding Pluronic F-68
• D. Increasing temperature

Hint:

When working with sensitive animal cells in bioreactors, consider using Pluronic F-68 to reduce shear damage and enhance cell viability.

Answer 1

The Correct Option is C

Pluronic F-68 is a surfactant that helps protect animal cells in culture by reducing shear stress, which is a common problem in bioreactors. It stabilizes cells and prevents damage caused by mechanical stress during stirring.