Question

Match LIST-I with LIST-II 

LIST-I (Technique)		LIST-II (Used for)
A.	Northern blotting	I.	To detect specific proteins in this sample of tissue homogenate
B.	Southern blotting	II.	Detection of specific post translation modification of proteins
C.	Western blotting	III.	To detect specific RNA molecule in mixture of RNA
D.	Eastern blotting	IV.	To detect specific DNA in a mix of samples

Choose the correct answer from the options given below: 
 

• A. A-II, B-III, C-IV, D-I
• B. A-III, B-IV, C-I, D-II
• C. A-III, B-IV, C-II, D-I
• D. A-I, B-II, C-III, D-IV

Hint:

Use the mnemonic {SNOW DROP} to remember the main blotting techniques: {S}outhern \(\rightarrow\) {D}NA {N}orthern \(\rightarrow\) {R}NA {O} \(\rightarrow\) {O} (Nothing) {W}estern \(\rightarrow\) {P}rotein

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
This question tests the knowledge of different blotting techniques used in molecular biology to detect specific macromolecules.
Step 2: Detailed Explanation:
B. Southern blotting: Named after its inventor, Edwin Southern, this technique is used IV. To detect specific DNA in a sample.
A. Northern blotting: This technique is analogous to Southern blotting but is used III. To detect specific RNA molecules, typically to study gene expression.
C. Western blotting: This technique uses antibodies to I. To detect specific proteins in a sample.
D. Eastern blotting: This is a less common technique used for the II. Detection of specific post-translational modification of proteins, such as glycosylation or phosphorylation.
Step 3: Final Answer:
The correct matches are A-III, B-IV, C-I, and D-II.