List of top Plant Biotechnology Questions asked in Common University Entrance Test

Match LIST-I with LIST-II 

LIST-ILIST-II
A.Sea weedsI.Isolation of DNA from gel
B.Staining of DNAII.Gel electrophoresis
C.ElutionIII.Source of agarose
D.Separation of DNA fragmentsIV.Ethidium bromide


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Match LIST-I with LIST-II 

LIST-I (Scientist)LIST-II (Landmark Discovery)
A.Van OverbeekI.Transgenic Bt-cotton
B.WhiteII.Introduced coconut water as a media component
C.WentIII.First synthetic plant tissue culture medium
D.MonsantoIV.First plant growth hormone i.e., IAA


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Match LIST-I with LIST-II 

LIST-ILIST-II
A.GynogenesisI.Callus culture
B.Culturing in liquid mediumII.Ovary culture
C.AndrogenesisIII.Suspension culture
D.Culturing on agar mediumIV.Pollen culture


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Match the LIST-I with LIST-II 

LIST-ILIST-II
A.AuxinI.Undifferentiated mass of cell
B.ProtoplastII.6-Furfuryl amino purine
C.CallusIII.Indole-3 Acetic Acid
D.CytokininIV.Pectinase


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Match LIST-I with LIST-II 

LIST-ILIST-II
A.ProtoplastI.Ability of cell to develop into a new plant
B.ExplantII.Unorganized mass of cells
C.TotipotencyIII.Naked cell
D.CallusIV.Any plant tissue used to regenerate new tissue/organ/plant in vitro


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Match the LIST-I with LIST-II 

LIST-ILIST-II
A.Karl ErekyI.Invented DNA 'Fingerprinting'
B.Joshua LederbergII.Coined the term 'Biotechnology'
C.Kary MullisIII.Discovered plasmids
D.Sir Alec JefferysIV.Developed polymerase chain reaction


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Match LIST-I with LIST-II 

LIST-I (Technique)LIST-II (Used for)
A.Northern blottingI.To detect specific proteins in this sample of tissue homogenate
B.Southern blottingII.Detection of specific post translation modification of proteins
C.Western blottingIII.To detect specific RNA molecule in mixture of RNA
D.Eastern blottingIV.To detect specific DNA in a mix of samples


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LIST-I (Organism)LIST-II (Use in Biotechnology)
A.Thermus aquaticusI.Cry proteins
B.Agrobacterium tumefaciensII.DNA polymerase
C.E. coli DH5aIII.epsps gene
D.Bacillus thuringiensisIV.DNA cloning


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Match LIST-I with LIST-II 

LIST-I (Biocontrol)LIST-II (Example)
A.BacteriumI.Bacillus thuringiensis
B.FungusII.Clostridium
C.InsectIII.Trichoderma
D.BiopesticideIV.Cotton aphid


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Match LIST-I with LIST-II 

LIST-ILIST-II
A.Biological nitrogen fixationI.Nitrobacter
B.Conversion of ammonia to nitriteII.Paracoccus
C.Conversion of nitrite to nitrateIII.Rhizobium
D.DenitrificationIV.Nitrosomonas


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Match LIST-I with LIST-II 

LIST-I (Culture Type)LIST-II (Use/Application)
A.Embryo cultureI.Somatic hybridization
B.Meristem cultureII.Production of haploids
C.Protoplast cultureIII.Shortening of breeding cycle
D.Anther cultureIV.Virus free plants


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Match LIST-I with LIST-II 

LIST-ILIST-II
A.Gene inhibitionI.Addition of functional gene to their genome to replace missing product
B.Gene editingII.Disarm the product of faulty gene
C.Gene targetingIII.CRISPR/Cas9
D.Gene Augmentation therapyIV.Replacement of non-functional gene with normal gene


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Match LIST-I with LIST-II 

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Arrange the steps in PEG induced protoplast fusion in correct sequence -
A. Treatment of protoplast mixture with 28-50% PEG for 15-30 minutes.
B. Protoplast aggregation.
C. Washing of protoplast (alkaline medium pH 9-10), and high Ca\textsuperscript{2+ concentration}
D. Selection of protoplasts of different strains / species.
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Which of the following will be present in the F\textsubscript{1 multicellular embryo, derived from a cross of female plant (A) with male plant B, through "bulbosum method"?
In general, the cytoplasmic male sterility (CMS) causing genes are transcribed in which plant cell organelle?
Select the correct combination of protoplast isolation enzyme and its most popular source -
Copies of DNA strands generated during a polymerase chain reaction are known as -
Which combination of strategies forms the basis of in vivo haploid induction technologies in plants?
A. induction of parthenogenesis
B. culture of anthers or ovules
C. use of paternal inducer lines
D. uniparental genome elimination
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Which gene in shoot apical meristem (SAM) negatively regulate WUS expression?
Chemical most widely used for chromosome doubling in haploid culture is-
What is CPW in protoplast culture method?
In cyanobacteria nitrogen fixation takes place in -
Arrange the following events in Western Blotting experiment in correct order:
A. Protein resolution by PAGE
B. Primary antibody binding
C. Transfer onto nitrocellulose membrane
D. Protein denaturation in loading dye.
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Synthetic seeds are produced by the encapsulation of somatic embryos with-