Question:
(iv) Why is insertional inactivation preferred over the use of selectable markers in cloning vectors?
(iv) Why is insertional inactivation preferred over the use of selectable markers in cloning vectors?
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Insertional inactivation is a biosafe method for identifying recombinants without relying on antibiotics.
Updated On: Jan 29, 2025
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Solution and Explanation
- Insertional inactivation allows for direct identification of recombinant colonies, as the disrupted gene leads to observable changes (e.g., loss of color or enzyme activity).
- It avoids the use of antibiotics, promoting biosafety.
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