Question

(iv) Why is insertional inactivation preferred over the use of selectable markers in cloning vectors?

Hint:

Insertional inactivation is a biosafe method for identifying recombinants without relying on antibiotics.

Answer 1

- Insertional inactivation allows for direct identification of recombinant colonies, as the disrupted gene leads to observable changes (e.g., loss of color or enzyme activity). 
- It avoids the use of antibiotics, promoting biosafety.