Mutation breeding involves using induced or spontaneous genetic changes (mutations) to develop new crop varieties. We need to identify which option was not primarily developed this way.
(1) Mango – Rosica from Peruvian variety Rosadodelca: 'Rosica' is known to be a bud sport (a type of somatic mutation) of a Peruvian mango variety. So, this is true for development through mutation.
(2) Papaya- Pusa Majesty from local type: 'Pusa Majesty' is a papaya variety developed by IARI, India. Its development typically involved conventional breeding methods such as selection from existing diverse 'local types' or through hybridization programs, rather than being primarily a product of induced or selected single mutation. So, this is likely not true for development through mutation as the primary method.
(3) Grape-Marvel Seedless from Delight: 'Marvel Seedless' grape arose as a bud sport (somatic mutation) from the 'Delight' variety. So, this is true for development through mutation.
(4) Banana- High gate from Gros Michel, Motta Poovan from Poovan: 'Highgate' is a dwarf mutant of 'Gros Michel' banana. 'Motta Poovan' is also recognized as a somatic mutant from the 'Poovan' banana. Many banana cultivars arise from such spontaneous mutations. So, this is true for development through mutation.
Based on this, 'Pusa Majesty' papaya stands out as the variety whose development is generally attributed to conventional breeding (selection/hybridization) rather than primarily mutation breeding.
Papaya- Pusa Majesty from local type