Question:
Identify A, B, and C in the diagram of pBR322 (A, B, and C are restriction sites):
Identify A, B, and C in the diagram of pBR322 (A, B, and C are restriction sites):
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Familiarizing yourself with restriction enzyme recognition sites and their locations on cloning vectors like pBR322 is essential for effective molecular biology experimentation.
Updated On: Mar 13, 2025
- BamH I, Hind III, Pst II
- BamH I, Hind III, Pst I
- Hind III, BamH I, Pst II
- Pst I, PVU II, BamH I
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The Correct Option is B
Solution and Explanation
In the plasmid map of pBR322, the restriction sites are crucial for molecular cloning:
A: BamH I, recognized for its specific cleavage pattern.
B: Hind III, commonly used for its clean cuts in DNA.
C: Pst I, another precise cutter in genetic engineering tasks.
These sites are strategically used to insert or remove DNA fragments for cloning purposes.
A: BamH I, recognized for its specific cleavage pattern.
B: Hind III, commonly used for its clean cuts in DNA.
C: Pst I, another precise cutter in genetic engineering tasks.
These sites are strategically used to insert or remove DNA fragments for cloning purposes.
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