Question

Arrange the following in correct order in polymerase chain reaction cycle: 
I Deannealing
II Denaturation
III Extension
IV Annealing 
 

• A. II - III - IV
• B. I - II - III
• C. I - III - IV
• D. II - IV - III

Hint:

Proper sequencing of PCR steps is critical for amplification efficiency and to avoid errors during DNA synthesis.

Answer 1

The Correct Option is D

Ordering PCR steps correctly. The correct sequence starts with Denaturation (II), followed by Annealing (IV), and concludes with Extension (III), describing the thermal cycle phases needed to amplify DNA.