Question

Arrange the following in correct order in polymerase chain reaction cycle: 
I Deannealing
II Denaturation
III Extension
IV Annealing 
 

• A. II - III - IV
• B. I - II - III
• C. I - III - IV
• D. II - IV - III

Hint:

Proper sequencing of PCR steps is critical for amplification efficiency and to avoid errors during DNA synthesis.

Answer 1

The Correct Option is D

To solve the problem, we need to arrange the steps of the polymerase chain reaction (PCR) cycle in the correct order.

1. Understanding the PCR Cycle:
Polymerase chain reaction (PCR) involves a series of temperature-dependent steps to amplify DNA. The basic steps are:

• Denaturation (II): The DNA is heated to separate the two strands of the double helix.
• Annealing (IV): The temperature is lowered to allow primers to bind to the target DNA sequences.
• Extension (III): The DNA polymerase extends the primers, synthesizing new DNA strands.

2. Analyzing the Correct Order:
The PCR steps follow a specific order:
Denaturation (II) occurs first, where the DNA is separated into single strands.
Annealing (IV) comes next, where primers bind to the separated strands.
Extension (III) occurs last, where the DNA polymerase extends the primers to synthesize new DNA strands.

Final Answer:
The correct order of steps is (D) II - IV - III.