An open reading frame (ORF) is a sequence of DNA that begins with a start codon and extends without interruption (no stop codon) to a stop codon, representing the coding sequence for a protein.

In the above represented plasmid an alien piece of DNA is inserted at the EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
Find the next two terms of the series:
The given series is: \( A, C, F, J, ? \).
(A) O
(B) U
(C) R
(D) V
Choose the correct answer from the options given below: