An open reading frame (ORF) is a sequence of DNA that begins with a start codon and extends without interruption (no stop codon) to a stop codon, representing the coding sequence for a protein.

In the above represented plasmid an alien piece of DNA is inserted at the EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
Match Fibre with Application.\[\begin{array}{|l|l|} \hline \textbf{LIST I} & \textbf{LIST II} \\ \textbf{Fibre} & \textbf{Application} \\ \hline \hline \text{A. Silk fibre} & \text{I. Fire retardant} \\ \hline \text{B. Wool fibre} & \text{II. Directional lustre} \\ \hline \text{C. Nomex fibre} & \text{III. Bulletproof} \\ \hline \text{D. Kevlar fibre} & \text{IV. Thermal insulation} \\ \hline \end{array}\]