Question

What is CPW in protoplast culture method?

• A. Cell and protoplast washing
• B. Cytosol and protoplasm washing
• C. Cell and protoplast waste
• D. Cell and proteolytic waste

Hint:

In technical fields like biotechnology, acronyms are common. For exams, it's helpful to create a small glossary of key acronyms like CPW, MS (Murashige and Skoog), PEG (Polyethylene glycol), etc.

Answer 1

The Correct Option is A

Step 1: Understanding the Concept:
This is a terminology question specific to the laboratory protocols used in plant protoplast isolation. It asks for the meaning of the acronym "CPW".
Step 2: Detailed Explanation:
In the process of isolating protoplasts, plant tissue is first treated with enzymes (like cellulase and pectinase) to digest the cell walls. After digestion, the resulting mixture contains protoplasts, undigested cells, cellular debris, and the enzyme solution.
It is crucial to wash the protoplasts to remove these enzymes and debris, which can be harmful. For this purpose, a specific salt solution is used. This solution is commonly referred to as CPW salt solution, where CPW stands for Cell and Protoplast Washing.
Step 3: Final Answer:
CPW stands for Cell and protoplast washing.