Question

Match LIST-I with LIST-II 

LIST-I (Organism)		LIST-II (Use in Biotechnology)
A.	Thermus aquaticus	I.	Cry proteins
B.	Agrobacterium tumefaciens	II.	DNA polymerase
C.	E. coli DH5a	III.	epsps gene
D.	Bacillus thuringiensis	IV.	DNA cloning

Choose the correct answer from the options given below: 
 

• A. A-I, B-II, C-IV, D-III
• B. A-II, B-III, C-IV, D-I
• C. A-II, B-I, C-IV, D-III
• D. A-IV, B-III, C-II, D-I

Hint:

Associate key organisms with their famous biotechnological product or use: {T. aquaticus} \(\rightarrow\) Taq Polymerase (PCR), {B. thuringiensis} \(\rightarrow\) Bt toxin (Cry proteins), {E. coli} \(\rightarrow\) Cloning Host, {Agrobacterium} \(\rightarrow\) Plant Transformation.

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
This question requires matching specific organisms with their key roles or products in the field of biotechnology.
Step 2: Detailed Explanation:
A. {Thermus aquaticus:} This is a thermophilic bacterium found in hot springs. It is the source of Taq polymerase, a heat-stable II. DNA polymerase that is essential for the Polymerase Chain Reaction (PCR). So, A matches II.
B. {Agrobacterium tumefaciens:} This bacterium is used as a natural vector to create transgenic plants. One of the most important genes transferred into crops using this method is the III. epsps gene, which confers resistance to the herbicide glyphosate. So, B matches III.
C. {E. coli DH5\(\alpha\):} This is a specific laboratory strain of E. coli that is optimized for use as a host organism in IV. DNA cloning (specifically, for maintaining and replicating plasmids). So, C matches IV.
D. {Bacillus thuringiensis:} This bacterium is the source of insecticidal proteins called I. Cry proteins, which are used as biopesticides and in the creation of insect-resistant GM crops (Bt crops). So, D matches I.
Step 3: Final Answer:
The correct set of matches is A-II, B-III, C-IV, D-I.