List of top Questions asked in CBSE CLASS XII

Name any two diseases that have been treated with whey.

Curd is advised to be administered with antibiotics. Why?

Name any two properties that can be manipulated using Protein Engineering.

What kind of information is available in UniProtKB and PDB databases?

During DNA sequencing, why is the autoradiogram read from bottom to top to arrive at the original sequence ?

Name the computer programmes that can perform gene prediction for bacterial and eukaryotic genomes.

How is BLAST used to analyse sequence similarity ? Explain.

Name three database retrieval tools available from the NCBI. What all do they allow us to access ?

Name two phytohormones which are generally added to prepare plant nutrient media.

Why is it advisable to use lesser number of steps for downstream processing?

Which ingredient has been used to fulfill vitamin requirement?

Which component in the given list is acting as the carbon source?

What are zymogens? How is chymotrypsinogen different from chymotrypsin?

Give the names of any three genes that are used as selectable markers in recombinant DNA technology. Also mention the trait/protein they specify.

Explain the steps involved in PCR amplification method.

Write whether Protein A is of intra or extra cellular origin.

Which step in the given purification scheme is metabolite specific?

Give any two drawbacks of animal cell culture in vitro.}

What are the advantages offered by creating a mouse model with gene knockout?

Compare the techniques of FISH with Microarray in terms of principle and applications.

Illustrate steps to show the construction of a recombinant DNA molecule.

Write about any two strategies available to enhance the production of secondary metabolites in plant genetic engineering.

Name the technique that helps to study the entire protein profile from a given cell type. Briefly explain the principle of this method.

Assertion (A): The exact chemical composition of complex microbial growth media is known.
Reason (R): Complex nutrient media is used when specific growth requirement of a microorganism is unknown.

Assertion (A): The regulation of pH is essential for survival of mammalian cells.
Reason (R): Animal cell cultures mostly make use of Bicarbonate – carbon dioxide buffering system to maintain pH.