Question

Why is DSS used instead of TMS as the \( ^1H \) chemical shift reference for a biological sample?

• A. \( \text{TMS can denature proteins} \)
• B. \( \text{The chemical shift of TMS is dependent on temperature} \)
• C. \( \text{DSS has a higher solubility in aqueous solution} \)
• D. \( \text{DSS is widely used in protein sample preparation} \)

Hint:

Selecting appropriate NMR reference compounds is crucial for achieving consistent and reliable chemical shift measurements.

Answer 1

The Correct Option is C

DSS (4,4-Dimethyl-4-silapentane-1-sulfonic acid) is commonly used as an internal standard in \( ^1H \) NMR spectroscopy for biological samples. Unlike TMS, which is highly hydrophobic, DSS is more soluble in aqueous solutions, preventing phase separation issues. Its solubility makes DSS ideal for protein and biomolecular NMR experiments, maintaining sample integrity.